e cadherin detection Search Results


e cadherin alphalisa kit  (Revvity)
86
Revvity e cadherin alphalisa kit
Effect of the cAMP pathway on E-cadherin and N-cadherin expression. A549 cells were treated with Br-cAMP (100 nm–1 μM) in the absence or presence of TGF-β1 (10 ng/ml) for 48 h. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (B) or N-cadherin (C) was quantified using <t>AlphaLISA</t> kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, vs. TGF-β1 alone.
E Cadherin Alphalisa Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e cadherin alphalisa kit/product/Revvity
Average 86 stars, based on 1 article reviews
e cadherin alphalisa kit - by Bioz Stars, 2026-03
86/100 stars
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ve cadherin  (Revvity)
92
Revvity ve cadherin
Effect of the cAMP pathway on E-cadherin and N-cadherin expression. A549 cells were treated with Br-cAMP (100 nm–1 μM) in the absence or presence of TGF-β1 (10 ng/ml) for 48 h. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (B) or N-cadherin (C) was quantified using <t>AlphaLISA</t> kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, vs. TGF-β1 alone.
Ve Cadherin, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ve cadherin/product/Revvity
Average 92 stars, based on 1 article reviews
ve cadherin - by Bioz Stars, 2026-03
92/100 stars
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antibody detecting e-cadherin  (Becton Dickinson)
90
Becton Dickinson antibody detecting e-cadherin
Effect of the cAMP pathway on E-cadherin and N-cadherin expression. A549 cells were treated with Br-cAMP (100 nm–1 μM) in the absence or presence of TGF-β1 (10 ng/ml) for 48 h. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (B) or N-cadherin (C) was quantified using <t>AlphaLISA</t> kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, vs. TGF-β1 alone.
Antibody Detecting E Cadherin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody detecting e-cadherin/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
antibody detecting e-cadherin - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Effect of the cAMP pathway on E-cadherin and N-cadherin expression. A549 cells were treated with Br-cAMP (100 nm–1 μM) in the absence or presence of TGF-β1 (10 ng/ml) for 48 h. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (B) or N-cadherin (C) was quantified using AlphaLISA kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, vs. TGF-β1 alone.

Journal: Frontiers in Pharmacology

Article Title: The A 2B Adenosine Receptor Modulates the Epithelial– Mesenchymal Transition through the Balance of cAMP/PKA and MAPK/ERK Pathway Activation in Human Epithelial Lung Cells

doi: 10.3389/fphar.2018.00054

Figure Lengend Snippet: Effect of the cAMP pathway on E-cadherin and N-cadherin expression. A549 cells were treated with Br-cAMP (100 nm–1 μM) in the absence or presence of TGF-β1 (10 ng/ml) for 48 h. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (B) or N-cadherin (C) was quantified using AlphaLISA kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are presented as mean values ± SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, vs. TGF-β1 alone.

Article Snippet: E-cad were quantified using E-cadherin AlphaLISA kit (PerkinElmer, #AL370), and N-cad were quantified using N-cadherin AlphaLISA kit (PerkinElmer, #AL379) following the manufacturer’s instructions.

Techniques: Expressing

Effect of cAMP and ERK1/2 phosphorylation blockade on A 2B AR-mediated changes in EMT markers. A549 cells were treated with BAY 60-6583 (100 nM) in the absence or presence of MRS 1706 (1 μM), PKA inhibitor H89 (100 nM) or MEK1/2 inhibitor PD98059 (1 μM) for 48 h. When indicated, the antagonist and inhibitor were applied 30 min before treatment with BAY 60-6583. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are the mean values ± SEM of three independent experiments, each performed in duplicate. (B) Cells were treated as above (and ERK 1/2 phosphorylation was evaluated after 30 min of treatment. The data were expressed as the percentage versus untreated cells (CTRL) set to 100%±SEM of at least three different experiments performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (C) or N-cadherin (D) were quantified using AlphaLISA kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are the mean values±SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Dunnett’s post hoc test or two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, ### P ≤ 0.001 vs. BAY alone. (E,F) Cells were treated as above and real-time RT-PCR analysis of EMT markers (E-cad, N-cad and Vimentin) (E) , and EMT transcription factors (ZEB1, Snail, Slug, TWIST) (F) was performed. The data were expressed as the fold change vs. the CTRL levels, which were set to 1 and are the mean values±SEM of three different experiments each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Dunnett’s post hoc test or two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01 vs. BAY alone.)

Journal: Frontiers in Pharmacology

Article Title: The A 2B Adenosine Receptor Modulates the Epithelial– Mesenchymal Transition through the Balance of cAMP/PKA and MAPK/ERK Pathway Activation in Human Epithelial Lung Cells

doi: 10.3389/fphar.2018.00054

Figure Lengend Snippet: Effect of cAMP and ERK1/2 phosphorylation blockade on A 2B AR-mediated changes in EMT markers. A549 cells were treated with BAY 60-6583 (100 nM) in the absence or presence of MRS 1706 (1 μM), PKA inhibitor H89 (100 nM) or MEK1/2 inhibitor PD98059 (1 μM) for 48 h. When indicated, the antagonist and inhibitor were applied 30 min before treatment with BAY 60-6583. (A) At the end of the treatment, cAMP production was quantified. The data were expressed as cAMP concentrations and are the mean values ± SEM of three independent experiments, each performed in duplicate. (B) Cells were treated as above (and ERK 1/2 phosphorylation was evaluated after 30 min of treatment. The data were expressed as the percentage versus untreated cells (CTRL) set to 100%±SEM of at least three different experiments performed in duplicate. Cells treated as above were lysed, and the expression of E-cadherin (C) or N-cadherin (D) were quantified using AlphaLISA kits. The data were expressed as E-cadherin and N-cadherin concentrations (ng/ml) and are the mean values±SEM of three independent experiments, each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Dunnett’s post hoc test or two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01, ### P ≤ 0.001 vs. BAY alone. (E,F) Cells were treated as above and real-time RT-PCR analysis of EMT markers (E-cad, N-cad and Vimentin) (E) , and EMT transcription factors (ZEB1, Snail, Slug, TWIST) (F) was performed. The data were expressed as the fold change vs. the CTRL levels, which were set to 1 and are the mean values±SEM of three different experiments each performed in duplicate. The significance of the differences was determined by one-way ANOVA, followed by Dunnett’s post hoc test or two-way ANOVA with Bonferroni correction and two-sided tests for multiple comparisons. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001 vs. the CTRL; # P ≤ 0.05, ## P ≤ 0.01 vs. BAY alone.)

Article Snippet: E-cad were quantified using E-cadherin AlphaLISA kit (PerkinElmer, #AL370), and N-cad were quantified using N-cadherin AlphaLISA kit (PerkinElmer, #AL379) following the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR